Journal: International Dental Journal

Article Title: The Effectiveness of Artificial Intelligence in Detection of Oral Cancer

doi: 10.1016/j.identj.2022.03.001

Figure Lengend Snippet: Characteristics of the included studies.

Article Snippet: 10 , Duran-Sierra et al USA , 57 patients for tissue biopsy examination of suspicious oral epithelial precancerous or cancerous lesions , Multispectral auto-fluorescence lifetime imaging , Learning machine: 1. Linear discriminant analysis, quadratic , SN = 94% SP = 74% F1 score = 0.85 , The model using spectral-only features was SVM. LOGREG was the best performing classification, WhileQDA was the best-performing model using time-resolved-only features..

Techniques: Biomarker Discovery, Plasmid Preparation, Imaging, Spectroscopy

Journal: Fertility and sterility

Article Title: Metabolic imaging using Fluorescence Lifetime Imaging Microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes

doi: 10.1016/j.fertnstert.2018.07.022

Figure Lengend Snippet: Comparative assessments of Clpp+/+ and Clpp−/− oocytes. A) Brightfield images show gross morphological features of oocytes, while NADH FLIM intensity images reflect the mitochondrial distribution. B) Obvious defects in Clpp−/− oocytes were not observed with brightfield, but fluorescence imaging revealed aberrant mitochondrial distributions in many oocytes. C) Metabolic imaging of Clpp+/+ (n=55) and Clpp−/− oocytes (n=52) detected highly significant differences in five of the eight parameters measured. Parameters are plotted in order of decreasing separation, with asterisks indicating the following p-values: (*: p<10−11) and (**: p<10−26) D) If the three most sensitive metabolic parameters (NADH long lifetime, NADH fraction engaged, and FAD long lifetime) are represented in a 3D plot, we can fit a plane that perfectly separates the two data sets. E) Clpp+/+ oocytes (n=46) and Clpp−/− oocytes (n=43) were lysed to obtain individual mtDNA copy number measurements. T-tests on mtDNA measurements showed only a marginally significant difference with a p-value of 0.042. Error bars represent standard errors.

Article Snippet: Disclosures: Tim Sanchez and Dan Needleman are co-founders and stake holders at LuminOva, Inc. Summary sentence: Mitochondrial dysfunction caused by reproductive aging (mild) and CLPP-deficiency (severe) is accurately detected by Fluorescence Lifetime Imaging Microscopy (FLIM).

Techniques: Fluorescence, Imaging

Journal: Fertility and sterility

Article Title: Metabolic imaging using Fluorescence Lifetime Imaging Microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes

doi: 10.1016/j.fertnstert.2018.07.022

Figure Lengend Snippet: Comparative assessments of oocytes from old (12-month) and young (3-month) mice. A,B) Neither brightfield images nor FLIM intensity images revealed obvious differences in morphology or mitochondrial distribution. 20um bar. C) Conversely, metabolic imaging measurements on the same oocytes effectively differentiated young (n=35) and old (n=29) groups, with four of the eight parameters showing significant differences. Parameters are plotted in order of decreasing separation, with asterisks indicating the following p-values: (*:p<0.02) and (**: p<10−3). P-values were not as low as with Clpp, the more severe case of metabolic dysfunction. D) If the three most sensitive metabolic parameters (NADH intensity NADH short lifetime, and NADH long lifetime) are represented in a 3D plot, we can draw a plane that effectively separates the two data sets; however, there is more overlap between the distributions than in the more extreme case of Clpp. E) mtDNA copy number measurements were taken on young (n=35) and old (n=29) oocytes, and no significant difference between the two groups was observed (p=0.14). Error bars represent standard errors.

Article Snippet: Disclosures: Tim Sanchez and Dan Needleman are co-founders and stake holders at LuminOva, Inc. Summary sentence: Mitochondrial dysfunction caused by reproductive aging (mild) and CLPP-deficiency (severe) is accurately detected by Fluorescence Lifetime Imaging Microscopy (FLIM).

Techniques: Imaging

Journal: Fertility and sterility

Article Title: Metabolic imaging using Fluorescence Lifetime Imaging Microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes

doi: 10.1016/j.fertnstert.2018.07.022

Figure Lengend Snippet: Evaluation of safety for varying photodoses of FLIM illumination. Top: Reactive oxygen species levels were measured via HC-DCFDA fluorescence (custom units). Significant differences between illuminated and non-illuminated embryos were not observed for any of the photodoses studied. Embryos exposed to 30mM H2O2 were measured as a positive control. Error standard error bars represent variation between individual embryo measurements. Bottom: Embryos were cultured on the microscope, and blastocyst development rates of illuminated embryos were compared to non-illuminated embryos in the same dish. Embryos cultured in a standard incubator were used as a control. FLIM illumination did not have any significant impact on blastocyst development rates. Standard error bars represent variation between experiment batches.

Article Snippet: Disclosures: Tim Sanchez and Dan Needleman are co-founders and stake holders at LuminOva, Inc. Summary sentence: Mitochondrial dysfunction caused by reproductive aging (mild) and CLPP-deficiency (severe) is accurately detected by Fluorescence Lifetime Imaging Microscopy (FLIM).

Techniques: Fluorescence, Positive Control, Cell Culture, Microscopy, Control

Journal: International Journal of Environmental Research and Public Health

Article Title: Autofluorescence Image-Guided Endoscopy in the Management of Upper Aerodigestive Tract Tumors

doi: 10.3390/ijerph20010159

Figure Lengend Snippet: Autofluorescence functioning based on different fluorescence characteristics of the tissues, which illuminates with different intensity (modified from Optical Imaging Lab-screening and diagnosis with light, Vancouver, Canada).

Article Snippet: For example, the usage of a fluorescence lifetime imaging technique in transoral robotic surgery enables the detection of oropharyngeal carcinoma in the setting of the management of metastatic carcinoma of unknown primary [ ].

Techniques: Fluorescence, Modification, Optical Imaging, Biomarker Discovery